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Effect of Maternal Nonalcoholic Fatty Liver Disease and Dietary Choline Status on Body Mass and Lipid Profile in Rat Offspring
- Joanna Mikołajczyk-Stecyna, Ewelina Żuk, Krzysztof Olszyński, Piotr Celichowski, Marcin Ruciński, Agata Chmurzynska
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- Journal:
- Proceedings of the Nutrition Society / Volume 79 / Issue OCE2 / 2020
- Published online by Cambridge University Press:
- 10 June 2020, E191
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Both maternal metabolic status and nutrition during pregnancy and lactation may have a programming effect on offspring metabolism. The aim of this study was to examine the role of the dietary choline supply during pregnancy and lactation in rat dams suffering from nonalcoholic fatty liver disease (NAFLD) on body weight and plasma lipid profile of the progeny.
The research protocol was approved by the local ethics committee. The study groups included the offspring of 1. healthy dams receiving choline during pregnancy and lactation (the control group); 2. NAFLD dams receiving choline during pregnancy and lactation (NN); 3. NAFLD dams receiving choline during pregnancy and a choline-deficient diet during lactation (ND); 4. NAFLD dams receiving a choline-deficient diet during pregnancy and a supply of choline during lactation (DN); and 5. NAFLD dams receiving a choline-deficient diet during both pregnancy and lactation (DD). Body mass and plasma lipid profile were assessed in male and female rats from each group on day 3 (3d), day 24 (24d), and day 90 (90d).
Body mass was significantly lower in the male offspring of the DD and DN groups than in the control group. Differences were observed at all times (3d: p = 0.0023; 24d: p < 0.0001; 90d: p < 0.0001). Moreover, body mass was significantly higher in the male offspring of the control group than in any other group. In the female progeny, body mass was higher in the control group than in the ND (24d: p < 0.0001; 90d: p = 0.0067) or NN (24d: p = 0.0058) groups.
Total plasma cholesterol concentration was higher in the 90d males of the control group than in the DD group (p = 0.0163) and in the 24d females of the NN group than in the ND group (p = 0.0495). In the 3d animals, LDL was higher (p = 0.0083) but HDL was lower (p = 0.0196) in male rats of the DD and DN groups than in the NN and ND groups. Neither age nor sex affected LDL levels. The plasma levels of triglycerides were not affected by the dietary regimen, sex, or age of the animals.
Maternal NAFLD and dietary choline status during pregnancy and lactation affect body mass and lipid profile in rat offspring, and the effects of maternal programming are more pronounced in male offspring than in female.
The project was financed by the National Science Centre, Poland (2016/21/D/NZ9/00360).
Fatty acid sensitivity, intake of high-fat foods, gene polymorphism, and body mass
- Agata Chmurzynska, Monika Młodzik-Czyżewska, Anna Malinowska, Grzegorz Galinski, Anna Radziejewska, Ewa Bulczak, Joanna Mikołajczyk-Stecyna
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- Journal:
- Proceedings of the Nutrition Society / Volume 79 / Issue OCE2 / 2020
- Published online by Cambridge University Press:
- 10 June 2020, E185
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- Article
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Taste perception is the main biological determinant of food choice. It has thus been hypothesized that fatty acid sensitivity may affect fat intake. The aim of this study was to examine the relationship between fatty acid sensitivity, frequency of consumption of high-fat products, polymorphism of genes encoding proteins involved in fat taste perception, and body mass.
421 people aged 20–40 were enrolled in Poznań, Poland. Body composition was measured using a Bod Pod. The frequency of consumption of high-fat foods was analyzed using an application for mobile devices based on the ecological momentary assessment approach. Food intake was assessed with dietary records. Salad dressings with varying concentrations of canola oil (from 2.5% to 40.0%) were used as stimuli to test fatty acid sensitivity. The individuals were then divided into groups with higher and lower fatty acid sensitivity. Lower sensitivity means that individuals were able to distinguish samples when the oil concentration exceeded 20%. Genotyping of rs1761667 (CD36), rs1573611 (FFAR1), and rs17108973 (FFAR4) was performed using TaqMan probes.
57% men and 61% women had higher sensitivity to fatty acids. Higher fatty acid sensitivity was associated with the GG genotype of CD36 (OR = 2.05, p < 0.05). People with different taste sensitivity did not differ in their frequency of consumption of high-fat foods or in their macronutrient intake. There was no association between body mass index (BMI) and fatty acid sensitivity, but people with BMI values below 25 more often ate high-fat products with favorable lipid profiles and less often ate meat high-fat products than subjects with BMI values over 25 (p < 0.001 and p < 0.05, respectively). There was no association between CD36 or FFAR4 genotype and fat intake or frequency of consumption of high-fat foods. People with the minor FFAR1 allele ate sweet high-fat products less often than major allele homozygotes (p < 0.05). Moreover, women ate high-fat products with favorable lipid profiles and sweet and savory high-fat products more frequently than men (p < 0.05, p < 0.001, and p < 0.01), but men ate meat high-fat products more frequently than women (p < 0.01).
Concluding, fatty acid sensitivity is associated with polymorphism of the CD36 gene. The frequency of consumption of high-fat foods depends on sex, but not on fatty acid sensitivity, BMI, or CD36 variants.
The project was financed by a National Science Centre award (decision number grant no. 2014/15/B/NZ9/02134).